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DIGE proteomics uses 2-dimensional gel electrophoresis (2D-DIGE) to analyze differential protein regulation between control and target samples. The technique utilizes three different Cyanine reactive dyes, each responding to a specific wavelength under appropriate excitation and emission conditions. Different samples are covalently labeled with different dyes, then mixed, and then fractionated in a single 2D-gel. With this set up, it is possible to compare hundreds of proteins in a single experiment under quantitative and reproducible conditions.

The technique has been successfully applied in many studies, including the following:

Brain tumors,

Alzheimer's disease,

Huntington's disease,

Lung tumors,

Cell-cycle regulation,

Memory and learning,

Visual orientation,

Protein modification,

Biomarker discovery.

The laboratory consists of two major workflows: 2D gel Electrophoresis (2DE) and Multidimensional Liquid Chromatography (MDLC). These services are open to all Duke University investigators. Laboratory personnel provide detailed assistance and consultation for sample preparation including specific protocols and methodologies with particular emphasis on differential protein analysis. Proteins of interest are identified by mass spectrometric analysis at the Duke Proteomics Facility.

To request pricing and services, please contact us at:

E-mail: npl@neuro.duke.edu

Phone: (919) 681 5835

URL: http://dige-proteomics.neuro.duke.edu/

Supporting Institutions
Department of Medicine: Division of Neurology
Department of Neurobiology
Duke University Medical Center

 

2D-DIGE:

2-Dimensional - Differential In Gel Expression analysis is a method for the study of differential protein expression using fluorescent dyes that label protein samples, which are then fractionated using 2D-gel electrophoresis. The general principle is summarized below, and explained in detail here.

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2D-HPLC
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