Multidimension Liquid Chromatography

 

 

Fluorescence Detector

 

The Fluorescence Detector aids in selectivity and can have a better detection limit for many compounds. Fluorescence is the property of some chemicals to absorb light and emit it nearly instantly at a longer wavelength. This detector has four options for excitation and one emission or vice versa.

Performance	10 fg anthracene LOD, Ex 250nm, Em 400 400nm H2O>400 Raman, Ex 350nm, Em 397nm, dark value 450nm, standard flow cell
Light Source	20W Xenon flash lamp normal mode: 20W economy mode: 5W
Pulse Frequency	365Hz for signal mode 73 Hz for economy mode
Excitation Monochromator	Concave holographic grating, F/1.6, blaze 300nm, range 200-700nm and zero-order, bandwidth 20nm
Photomultiplier	200-600nm, exchangeable
Reference system	Inline excitation measurement
Time Programming	Up to four signals, response time, PMT gain, baseline behavior (append, free, zero), spectral parameters
Spectral Acquisition	Excitation or emission spectra, scan speed 28ms per datapoint
Step Size	1-20nm
Spectra Storage	All
Wavelength repeatability	±0.2nm
Wavelength Accuracy	±3nm
Flow Cells	Standard: 8µl volume, 20 bar (2MPa) pressure maximum, quartz
Environment	0-40°C constant temperature at <95% humidity (non-condensing)